Goal:

Using a highly accessible style and format these lectures and labworks will provide an understanding of the underlying principles, benefits, and limitations of optical techniques currently used in biology.

Content:

Without relying on complex mathematics we are going to address basic concepts in imaging such as contrasting techniques, fluorescence and also explores advanced techniques such as quantitative fluorescence, three-dimensional imaging, nonlinear microscopy. Of course we'll also have to spend some time on multiple appendices on cell handling, labeling, and image manipulation.

Prerequisites:

basics of optics, molecular biology